Question

Given below are four statements pertaining to separation of DNA fragments using Gel electrophoresis. Identify the incorrect statements.
(i) DNA is negatively charged molecule and so it is loaded on gel towards the Anode terminal.
(ii) DNA fragments travel along the surface of the gel whose concentration does not affect movement of DNA.
(iii) Smaller the size of DNA fragment, larger is the distance it travels through it.
(iv) Pure DNA can be visualised directly by exposing to UV radiation.
Choose correct answer from the options given below:

A. (i), (ii) and (iv)  
B. (i), (iii) and (iv)
C. (i), (ii) and (iii)
D. (ii), (iii) and (iv)
Answer :   (i), (ii) and (iv)

Releted MCQ Question on
Zoology >> Biotechnology: Principles and Processes

Releted Question 1

In gel electrophoresis, separated DNA fragments can be visualized with the help of

A. Ethidium bromide in UV radiation
B. Acetocarmine in UV radiation
C. Ethidium bromide in infrared radiation
D. Acetocarmine in bright blue light
Releted Question 2

Bt cotton variety that was developed by the introduction of toxin gene of Bacillus thuringiensis (Bt) is resistant to

A. Fungal diseases
B. Plant nematodes
C. Insect predators
D. Insect pests
Releted Question 3

Identify the wrong statement with regard to Restriction Enzymes.

A. They cut the strand of DNA at palindromic sites
B. They are useful in genetic engineering
C. Sticky ends can be joined by using DNA ligases
D. Each restriction enzyme functions by inspecting the length of a DNA sequence
Releted Question 4

The specific palindromic sequence which is recognized by EcoRl is

A. 5' - GGAACC - 3'
3' - CCTTGG - 5'
B. 5' - CTTAAG - 3'
3' - GAATTC - 5'
C. 5' - GGATCC - 3'
3' - CCTAGG - 5'
D. 5' - GAATTC - 3'
3' - CTTAAG - 5'

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Biotechnology: Principles and Processes


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