1. The DNA fragments separated on an agarose gel can be visualised after staining with:

A Acetocarmine
B Aniline blue
C Ethidium bromide
D Bromophenol blue
Answer :   Ethidium bromide
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2. In bacterial chromosomes, the nucleic acid polymers are

A linear DNA molecule
B circular DNA molecule
C of two types-DNA and RNA
D linear RNA molecule
Answer :   circular DNA molecule
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3. DNA or RNA segment tagged with a radioactive molecule is called

A Vector
B Probe
C Clone
D Plasmid
Answer :   Probe
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4. What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis?

A The smaller the fragment size, the farther it moves
B Positively charged fragments move to farther end
C Negatively charged fragments do not move
D The larger the fragment size, the farther it moves
Answer :   The smaller the fragment size, the farther it moves
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5. The correct order of steps in Polymerase Chain Reaction (PCR) is

A Extension, Denaturation, Annealing
B Annealing, Extension, Denaturation
C Denaturation, Annealing, Extension
D Denaturation, Extension, Annealing
Answer :   Denaturation, Annealing, Extension
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6. Introduction of food plants developed by genetic engineering is not desirable because

A economy of developing countries may suffer
B these products are less tasty as compared to the already existing products
C this method is costly
D there is danger of entry of viruses and toxins with introduced crop
Answer :   there is danger of entry of viruses and toxins with introduced crop
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7. What is antisense technology?

A A cell displaying a foreign antigen used for synthesis of antigens
B Production of somaclonal variants in tissue cultures
C When a piece of RNA that is complementary in sequence is used to stop expression of a specific gene
D RNA polymerase producing DNA
Answer :   When a piece of RNA that is complementary in sequence is used to stop expression of a specific gene
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8. Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes?

A Retrovirus
B Ti plasmid
C pBR 322
D $$\lambda $$ phage
Answer :   Retrovirus
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9. The plasmid

A helps in respiration
B genes found inside nucleus
C is a component of cell wall of bacteria
D is the genetic part in addition to DNA in micro-organisms
Answer :   is the genetic part in addition to DNA in micro-organisms
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10. Bt cotton variety that was developed by the introduction of toxin gene of Bacillus thuringiensis (Bt) is resistant to

A Fungal diseases
B Plant nematodes
C Insect predators
D Insect pests
Answer :   Insect pests
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