Biotechnology: Principles and Processes MCQ Questions & Answers in Zoology

51. Which one of the following is used as vector for cloning genes into higher organisms?

A Baculovirus

B Salmonella typhimurium

C Rhizopus nigricans

D Retrovirus

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52. The linking of antibiotic resistance gene with the plasmid vector became possible with

A DNA ligase

B Endonucleases

C DNA polymerase

D Exonucleases

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53. Organelle/organoid involved in genetic engineering is

A plasmid

B mitochondrion

C golgi apparatus

D lomasome

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54. A single strand of nucleic acid tagged with a radioactive molecule is called :

A Vector

B Selectable marker

C Plasmid

D Probe

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55. In genetic engineering, the antibiotics are used

A as selectable markers

B to select healthy vectors

C to keep the cultures free of infection

D as sequences from where replication starts

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56. Polyethylene glycol method is used for

A biodiesel production

B seedless fruit production

C energy production from sewage

D gene transfer without a vector

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57. Given below are four statements pertaining to separation of DNA fragments using Gel electrophoresis. Identify the incorrect statements.
(i) DNA is negatively charged molecule and so it is loaded on gel towards the Anode terminal.
(ii) DNA fragments travel along the surface of the gel whose concentration does not affect movement of DNA.
(iii) Smaller the size of DNA fragment, larger is the distance it travels through it.
(iv) Pure DNA can be visualised directly by exposing to UV radiation.
Choose correct answer from the options given below:

A (i), (ii) and (iv)

B (i), (iii) and (iv)

C (i), (ii) and (iii)

D (ii), (iii) and (iv)

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58. DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by :

A Polymerase chain reaction

B Electrophoresis

C Restriction mapping

D Centrifugation

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59. Which one is a true statement regarding DNA polymerase used in PCR

A It is used to ligate introduced DNA in recipient cell

B It serves as a selectable marker

C It is isolated from a virus

D It remains active at high temperature

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60. Two bacteria found to be very useful in genetic engineering experiments are

A Escherichia and Agrobacterium

B Nitrobacter and Azotobacter

C Rhizobium and Diplococcus

D Nitrosomonas and Klebsiella

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51. Which one of the following is used as vector for cloning genes into higher organisms?

52. The linking of antibiotic resistance gene with the plasmid vector became possible with

53. Organelle/organoid involved in genetic engineering is

54. A single strand of nucleic acid tagged with a radioactive molecule is called :

55. In genetic engineering, the antibiotics are used

56. Polyethylene glycol method is used for

58. DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by :

59. Which one is a true statement regarding DNA polymerase used in PCR

60. Two bacteria found to be very useful in genetic engineering experiments are

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