41. What is true for plasmid?

A Plasmids are widely used in gene transfer
B These are found in virus
C Plasmid contains gene for vital activities
D These are main part of chromosome
Answer :   Plasmids are widely used in gene transfer
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42. Stirred tank bioreactors have been designed for

A addition of preservatives to the product
B purification of the product
C ensuring anaerobic conditions in the culture vessel
D availability of oxygen throughout the process
Answer :   availability of oxygen throughout the process
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43. The process of separation and purification of expressed protein before marketing is called:

A Downstream processing
B Bioprocessing
C Postproduction processing
D Upstream processing
Answer :   Downstream processing
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44. The cutting of DNA at specific locations became possible with the discovery of

A Probes
B Selectable markers
C Ligases
D Restriction enzymes
Answer :   Restriction enzymes
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45. In bacteria, plasmid is

A extra - chromosomal material
B main DNA
C non-functional DNA
D repetitive gene
Answer :   extra - chromosomal material
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46. Which one of the following represents a palindromic sequence in DNA?

A 5' - GAATTC - 3'
3' - CTTAAG - 5'
B 5' - CCAATG - 3'
3' - GAATCC - 5'
C 5' - CATTAG - 3'
3' - GATAAC - 5'
D 5' - GATACC - 3'
3' - CCTAAG - 5'
Answer :   5' - GAATTC - 3'
3' - CTTAAG - 5'
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47. Which of the following is not a feature of the plasmids?

A Independent replication
B Circular structure
C Transferable
D Single - stranded
Answer :   Single - stranded
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48. Which one of the following techniques made it possible to genetically engineer living organism

A Recombinant DNA techniques
B X-ray diffraction
C Heavier isotope labelling
D Hybridization
Answer :   Recombinant DNA techniques
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49. A gene whose expression helps to identify transformed cell is known as :

A Vector
B Plasmid
C Structural gene
D Selectable marker
Answer :   Selectable marker
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50. In gel electrophoresis, separated DNA fragments can be visualized with the help of

A Ethidium bromide in UV radiation
B Acetocarmine in UV radiation
C Ethidium bromide in infrared radiation
D Acetocarmine in bright blue light
Answer :   Ethidium bromide in UV radiation
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