Biotechnology: Principles and Processes MCQ Questions & Answers in Zoology

31. The specific palindromic sequence which is recognized by EcoRl is

A 5' - GGAACC - 3'
3' - CCTTGG - 5'

B 5' - CTTAAG - 3'
3' - GAATTC - 5'

C 5' - GGATCC - 3'
3' - CCTAGG - 5'

D 5' - GAATTC - 3'
3' - CTTAAG - 5'

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32. During the process of isolation of DNA, chilled ethanol is added to

A Remove proteins such as histones

B Precipitate DNA

C Break open the cell to release DNA

D Facilitate action of restriction enzymes

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33. A selectable marker is used to :

A Mark a gene on a chromosome for isolation using restriction enzyme

B Help in eliminating the non-transformants, so that the transformants can be regenerated

C Identify the gene for a desired trait in an alien organism

D Select a suitable vector for transformation in a specific crop

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34. Biolistics (gene-gun) is suitable for

A DNA finger printing

B Disarming pathogen vectors

C Transformation of plant cells

D Constructing recombinant DNA by joining with vectors

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35. In vitro clonal propagation in plants is characterized by:

A PCR and RAPD

B Northern blotting

C Electrophoresis and HPLC

D Microscopy

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36. Which one of the following equipments is essentially required for growing microbes on a large scale, for industrial production of enzymes?

A BOD incubator

B Sludge digester

C Industrial oven

D Bioreactor

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37. Restriction endonucleases are enzymes which

A make cuts at specific positions within the DNA molecule

B recognize a specific nucleotide sequence for binding of DNA ligase

C restrict the action of the enzyme DNA polymerase

D remove nucleotides from the ends of the DNA molecule

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38. Which one of the following is commonly used in transfer of foreign DNA into crop plants?

A Meloidogyne incognita

B Agrobacterium tumefaciens

C Penicillium expansum

D Trichoderma harzianum

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39. Agarose extracted from sea weeds finds use in:

A Spectrophotometry

B Tissue culture

C PCR

D Gel electrophoresis

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40. Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme?

A 5'.............CGTTCG.............3'
3'.............ATGGTA.............5'

B 5'.............GATATG.............3'
3'.............CTACTA.............5'

C 5'.............GAATTC.............3'
3'.............CTTAAG.............5'

D 5'.............CACGTA.............3'
3'.............CTCAGT.............5'

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31. The specific palindromic sequence which is recognized by EcoRl is

32. During the process of isolation of DNA, chilled ethanol is added to

33. A selectable marker is used to :

34. Biolistics (gene-gun) is suitable for

35. In vitro clonal propagation in plants is characterized by:

36. Which one of the following equipments is essentially required for growing microbes on a large scale, for industrial production of enzymes?

37. Restriction endonucleases are enzymes which

38. Which one of the following is commonly used in transfer of foreign DNA into crop plants?

39. Agarose extracted from sea weeds finds use in:

40. Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme?

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