21.
For transformation, micro-particles coated with DNA to be bombarded with gene gun are made up of :
A
Silver or Platinum
B
Platinum or Zinc
C
Silicon or Platinum
D
Gold or Tungsten
Answer :
Gold or Tungsten
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22.
An enzyme catalysing the removal of nucleotides from ends of DNA is :
A
Protease
B
DNA ligase
C
Endonuclease
D
Exonuclease
Answer :
Exonuclease
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23.
Chromosomes in a bacterial cell can be 1 - 3 in number and
A
can be circular as well as linear within the same cell
B
are always circular
C
are always linear
D
can be either circular or linear, but never both within the same cell
Answer :
are always circular
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24.
PCR and Restriction Fragment Length Polymorphism are the methods for :
A
Study of enzymes
B
Genetic transformation
C
DNA sequencing
D
Genetic Fingerprinting
Answer :
Genetic Fingerprinting
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25.
Choose the correct pair from the following
A
Polymerases - Break the DNA into fragments
B
Nucleases - Separate the two strands of DNA
C
Exonucleases - Make cuts at specific positions within DNA
D
Ligases - Join the two DNA molecules
Answer :
Polymerases - Break the DNA into fragments
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26.
Gel electrophoresis is used for
A
cutting of DNA into fragments
B
separation of DNA fragments according to their size
C
construction of recombinant DNA by joining with cloning vectors
D
isolation of DNA molecule
Answer :
separation of DNA fragments according to their size
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27.
The two antibiotic resistance genes on vector pBR322 are for
A
Tetracycline and Kanamycin
B
Ampicillin and Tetracycline
C
Ampicillin and Chloramphenicol
D
Chloramphenicol and Tetracycline
Answer :
Ampicillin and Tetracycline
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28.
Genes of interest can be selected from a genomic library by using
A
Restriction enzymes
B
Cloning vectors
C
DNA probes
D
Gene targets
Answer :
DNA probes
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29.
The DNA molecule to which the gene of interest is integrated for cloning is called:
A
Vector
B
Template
C
Carrier
D
Transformer
Answer :
Vector
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30.
The sequence that controls the copy number of the linked DNA in the vector, is termed
A
Ori site
B
Palindromic sequence
C
Recognition site
D
Selectable marker
Answer :
Ori site
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