11.
Which of the following is a restriction endonuclease?
A
Hind II
B
Protease
C
DNase I
D
RNase
Answer :
Hind II
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12.
DNA precipitation out of a mixture of biomolecules can be achieved by treatment with:
A
Isopropanol
B
Chilled ethanol
C
Methanol at room temperature
D
Chilled chloroform
Answer :
Chilled ethanol
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13.
Commonly used vectors for human genome sequencing are:
A
T-DNA
B
BAC and YAC
C
Expression Vectors
D
kT/A Cloning Vectors
Answer :
BAC and YAC
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14.
Which vector can clone only a small fragment of DNA?
A
Bacterial artificial chromosome
B
Yeast artificial chromosome
C
Plasmid
D
Cosmid
Answer :
Plasmid
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15.
The taq polymerase enzyme is obtained from
A
Thermus aquaticus
B
Thiobacillus ferroxidans
C
Bacillus subtilis
D
Pseudomonas putida
Answer :
Thermus aquaticus
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16.
Following statements describe the characteristics of the enzyme Restriction Endonuclease. Identify the incorrect statement.
A
The enzyme cuts DNA molecule at identified position within the DNA
B
The enzyme binds DNA at specific sites and cuts only one of the two strands
C
The enzyme cuts the sugar-phosphate backbone at specific sites on each strand
D
The enzyme recognizes a specific palindromic nucleotide sequence in the DNA
Answer :
The enzyme binds DNA at specific sites and cuts only one of the two strands
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17.
Identify the wrong statement with regard to Restriction Enzymes.
A
They cut the strand of DNA at palindromic sites
B
They are useful in genetic engineering
C
Sticky ends can be joined by using DNA ligases
D
Each restriction enzyme functions by inspecting the length of a DNA sequence
Answer :
Sticky ends can be joined by using DNA ligases
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18.
The colonies of recombinant bacteria appear white in contrast to blue colonies of non- recombinant bateria because of:
A
Insertional inactivate of alphaga- lactosidase in non-recombinant bacteria
B
Insertional inactivation of alpha- galactosidase in recombinant bacteria
C
Inactivation of glycosidase enzyme in recombinant bacteria
D
Non-recombinant bacteria containing beta-galactosidase
Answer :
Non-recombinant bacteria containing beta-galactosidase
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19.
There is a restriction endonuclease called EcoRI. What does "co" part in it stand for?
A
colon
B
coelom
C
coenzyme
D
coli
Answer :
coli
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20.
The plasmids pesent in the bacterial cells are
A
circular double helical DNA molecules
B
circular double helical RNA molecules
C
linear double helical DNA molecules
D
linear double helical RNA molecules
Answer :
circular double helical DNA molecules
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